Gene-edited mice

Strain name

Strain Background

Target gene/Cre type

Application Notes

​ B6.Cg-Tg(Alb-cre)21Mgn/J

C57BL/6

Alb (albumin promoter drives Cre)

Liver-specific gene knockout for studying metabolic diseases, liver cancer, or drug hepatotoxicity.

​ B6.Cg-Tg(Nes-cre)1Kln/J

C57BL/6

Nestin (Neural Stem Cell/Progenitor Cell Cre)

Central nervous system (neurons, glial cells) conditional knockout for neurodevelopmental or degenerative disease research.

​ B6.129S4-Cd19tm1(cre)Cgn/J

C57BL/6 × 129S4

CD19 (B cell-specific Cre)

B cell gene knockout to study antibody production, autoimmune disease, or lymphoma.

​ B6.Cg-Tg(Myh6-cre/ERT2)1Jmk/J

C57BL/6

Myh6 (α-myosin heavy chain, tamoxifen-inducible Cre)

Cardiomyocyte-specific inducible knockout for use in cardiac regeneration or heart failure models.

​ B6;129S-Tg(Vil1-cre)997Gum/J

C57BL/6 × 129S

Vil1 (intestinal epithelial cell-specific Cre)

Intestinal epithelial gene knockout for inflammatory bowel disease (IBD) or gut microbiota interaction studies.

​ B6.Cg-Tg(Tek-cre)1Ywa/J

C57BL/6

Tek (Tie2, Endothelial Cre)

Conditional knockout of endothelial cells to study angiogenesis, tumor vascularization, or atherosclerosis.

​ B6.Cg-Tg(Camk2a-cre)T29-1Stl/J

C57BL/6

Camk2a (forebrain neuron-specific Cre)

Gene knockout of excitatory neurons (such as hippocampus and cortex) is used to study learning and memory or epilepsy mechanisms.

​ B6.129P2-Lyz2tm1(cre)Ifo/J

C57BL/6 × 129P2

Lyz2 (lysozyme, myeloid cell Cre)

Specific knockout of macrophages and neutrophils for infection immunity or tumor microenvironment research.

​ B6.Cg-Tg(Sox2-cre)1Amc/J

C57BL/6

Sox2 (Epithelial Stem Cell Cre)

Gene knockout of epithelial stem cells in the skin, lung, intestine, etc. to study tissue regeneration or cancer stem cells.

​ B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J

C57BL/6

UBC (ubiquitin C promoter, tamoxifen-inducible Cre)

Systemic knockout (requires crossing with floxed mice) is used to study gene function in adult animals.

​ B6;129S-Gt(ROSA)26Sortm1(cre/ERT2)Tmj/J

C57BL/6 × 129S

ROSA26 (tamoxifen-inducible Cre)

Systemic controllable knockout, suitable for multi-tissue gene function research (needs to be used in combination with tissue-specific promoters).

​ B6;129S6-Chattm1(cre)Lowl/J

C57BL/6 × 129S6

ChAT (Choline Acetyltransferase, Cholinergic Neuron Cre)

Cholinergic neuron-specific knockout for Alzheimer's disease or autonomic nervous system function research.

Strain name

Strain Background

Target Gene

Application Notes

​ B6.129S2-Trp53tm1Tyj/J

C57BL/6 × 129S2

Trp53 (p53)

Spontaneous lymphoma, sarcoma (6-12 months old), increased tumor susceptibility. Cancer occurrence mechanism, chemotherapy drug screening

​ B6.129P2-Apoetm1Unc/J

C57BL/6 × 129P2

ApoE (Apolipoprotein E)

High-fat diet induces atherosclerotic plaques (12-16 weeks) and lipid metabolism disorders. Cardiovascular disease, Alzheimer's disease

​ B6.129S1-Il10tm1Cgn/J

C57BL/6 × 129S1

IL-10

Spontaneous colitis (3-6 months of age), immune dysregulation. Inflammatory bowel disease (IBD), immune tolerance

​ B6.129S4-Cd19tm1(cre)Cgn/J

C57BL/6 × 129S4

CD19

B cell development is blocked, there are no mature B cells, and humoral immunity is defective. B cell function, autoimmune disease

​ B6.Cg-Lepob/J

C57BL/6

Leptin

Obesity, hyperglycemia, insulin resistance (from 4 weeks of age), metabolic syndrome phenotype. Research on the mechanism of type 2 diabetes and obesity

​ B6.129S7-Rag1tm1Mom/J

C57BL/6 × 129S7

Rag1

T/B lymphocyte deficiency, severe combined immunodeficiency (SCID). Immune reconstruction, humanized tumor transplantation (PDX)

​ B6.129S-Tg(APP)Swe/J

C57BL/6 × 129S

APP (Swedish mutation)

Overexpression of Aβ, the appearance of amyloid plaques at 6 months of age, and cognitive decline. Pathological mechanism of Alzheimer's disease

​ B6.129S-Tg(SOD1)G93A/J

C57BL/6 × 129S

SOD1 (G93A mutation)

Motor neuron degeneration and hind limb paralysis (3-4 months of age) mimics amyotrophic lateral sclerosis (ALS).
Neurodegenerative diseases, drug testing

​ B6.129S2-Ins2tm1Tyj/J

C57BL/6 × 129S2

Ins2 (Insulin 2)

Postnatal hyperglycemia, β-cell failure, simulating type 1 diabetes. Diabetes mechanism, islet transplantation research

​ B6.129S4-Gt(ROSA)26Sortm1Hjf/J

C57BL/6 × 129S4

ROSA26 (LacZ reporter)

Systemic LacZ expression for tracking gene knock-in or cell lineage. Reporter gene system, cell fate mapping

Strain name

Strain Background

Cre type/promoter

Target cells/tissues

Application Scenario

B6.Cg-Tg(CMV-cre)1Cgn/J

C57BL/6

Cre driven by the constitutive CMV promoter

Widely expressed throughout the body

Basic research (needs to be crossed with floxed mice to achieve systemic knockout), but may result in missed cuts or embryonic lethality.

B6.Cg-Tg(Sox2-cre)1Amc/J

C57BL/6

Sox2 (Epithelial Stem Cell Cre)

Epithelial stem cells such as skin, lung, and intestine

Study tissue regeneration, epithelial carcinogenesis or stem cell function.

​B6.Cg-Tg(Alb-cre)21Mgn/J

C57BL/6

Alb (albumin promoter drives Cre)

Hepatocytes

Liver-specific genetic manipulation (e.g. liver cancer models, metabolic diseases).

​B6;129S-Gt(ROSA)26Sortm1(cre/ERT2)Tmj/J

C57BL/6 × 129S

Insertion of tamoxifen-inducible Cre-ERT2 at the ROSA26 locus

Systemically controllable (requires tamoxifen induction)

Temporally and spatially specific gene knockout in adult animals avoids developmental lethal effects.

B6.Cg-Tg(Nes-cre)1Kln/J

C57BL/6

Nestin (Neural Stem Cell/Progenitor Cell Cre)

Central nervous system (neurons, glial cells)

Neurodevelopmental, glioma, or neurodegenerative disease research.

​B6.Cg-Tg(Myh6-cre/ERT2)1Jmk/J

C57BL/6

Myh6 (α-myosin heavy chain, inducible Cre)

Cardiomyocytes

Cardiac disease models (e.g., post-myocardial infarction repair, myocardial fibrosis).

​B6.129S4-Cd19tm1(cre)Cgn/J

C57BL/6 × 129S4

CD19 (B cell-specific Cre)

B lymphocytes

Research on immune response, B-cell lymphoma or autoantibody related diseases.

​B6.Cg-Tg(Tek-cre)1Ywa/J

C57BL/6

Tek (Tie2, Endothelial Cre)

Endothelial cells

Angiogenesis, tumor vascularization or atherosclerosis research.

B6.Cg-Tg(Lyz2-cre)1Ifo/J

C57BL/6

Lyz2 (lysozyme, myeloid cell Cre)

Macrophages, neutrophils

Research on infection immunity and tumor-associated macrophage (TAM) function.

​B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J

C57BL/6

Ubiquitin C promoter drives tamoxifen-inducible Cre

Systemic induction (requires tamoxifen)

Study the function of genes in adulthood (avoiding interference during development).