Synthesis and Functionality: 740 YP is an artificially designed phosphorylated peptide that mimics the key tyrosine phosphorylation site in the PI3K (phosphatidylinositol 3-kinase) signaling pathway and is used to activate the PI3K/Akt/mTOR pathway. Its sequence contains phosphorylated tyrosine (pY) residues that can directly bind to the PI3K regulatory subunit and promote downstream signal transduction.
In vitro studies: • In cell lines (such as HEK293, MCF-7), 740 YP induces Akt phosphorylation by activating PI3K, thereby enhancing cell survival, proliferation and metabolic regulation. • Used to study the mechanism of PI3K-dependent signaling pathways, or as a positive control to verify the effects of inhibitors (such as LY294002).
In vivo studies: • In animal models (such as tumor-transplanted mice), local injection can transiently activate the PI3K pathway in target tissues and is used to explore the molecular mechanisms of cancer, immune or metabolic diseases.
Application areas: • Cancer research (tumor growth, drug resistance mechanisms) • Metabolic diseases (regulation of insulin signaling pathway) • Immunology (T/B cell activation studies)
After receiving the product, please store it in accordance with the conditions recommended in the instructions. Unless otherwise specified, the relevant products are all lyophilized powders. Since trace amounts of polypeptides are deposited in the tube during the lyophilization process, forming a very thin or invisible polypeptide layer, Before capping the tube, we recommend centrifuging at about 8,000-12,000 g for 10-30 seconds to allow the peptides attached to the tube cap or tube wall to accumulate at the bottom of the tube.
Please refer to relevant literature for the specific optimal working concentration, or explore and optimize through experiments based on the purpose of the experiment and specific cells and animals.
Our company can provide customized synthesis of high-polypeptide, virtual screening of compounds, Animal disease model construction, Drug safety verification, Application technology support for gene editing animals, etc. For specific application consultation, please contact the sales department.
We look forward to your inquiries:
Sales Department 1: Manager Han Mobile phone: 18701856899
