Dnp-PLGLWAr-NH₂

Basic Description

Structure and function ​:
This peptide is a fluorescence quenching protease substrate, and the sequence design contains the following functional modules:
1. ​​Quencher ​：N-terminal 2,4-dinitrophenyl (Dnp) acts as a fluorescence quencher.
2. ​​Restriction sites​ ​：PLGLWA is a specific recognition sequence for matrix metalloproteinases (MMP-2/9) and can be cleaved to release C-terminal fluorescent signals.
3. ​​D-Arginine (r)​ ​：Enhance substrate stability and enzyme cleavage specificity.
4. ​​C-terminal amidation​ ​：Improve the stability of peptides.

​ ​Research Applications​ ​:
• ​In vitro testing ​：Real-time monitoring of MMP-2/9 enzyme activity (fluorescence recovery after cleavage, Ex/Em=320 nm/405 nm).
• ​​Drug Screening​ ​：Evaluation of the efficacy of MMP inhibitors or activators.
• ​​Tumor Models ​：Study the relationship between MMP activity and invasion and metastasis in the tumor microenvironment.

Complete sequence

Dnp-Pro-Leu-Gly-Leu-Trp-Ala-Arg-NH₂

Molecular formula

C₅₃H₇₈N₁₆O₁₃S

Molecular weight

1223.34

purity

>95%

form

Lyophilized powder

Storage conditions

Store in a dry place at -20°C