Hexa-His

Basic Description

​Synthesis and Functionality​ ​:
Hexa-His peptide is a short peptide composed of 6 consecutive histidine residues (His-His-His-His-His), which is widely used in affinity purification (such as nickel/cobalt ion metal chelate chromatography) and detection of recombinant proteins. Its core functions include:
1. ​​High affinity binding​ ​：Rapid purification is achieved by binding to Ni²⁺/Co²⁺ through the imidazole group of the histidine side chain.
2. ​​Gentle elution​ ​：Using competitive eluents (such as imidazole or low pH buffer) can efficiently recover the target protein and maintain biological activity.
3. ​Versatility ​：Applicable to various expression systems such as prokaryotes and eukaryotes, with minimal effect on protein folding.

​ ​Application Areas​ ​:
• Recombinant protein purification (IMAC chromatography)
• Western Blot/ELISA test (Anti-His antibody recognition)
• Protein immobilization (biosensor or chip preparation)

Complete sequence

His-His-His-His-His-His

Simplify sequence

HHHHHH

Molecular formula

C₃₀H₄₄N₁₂O₆

Molecular weight

668.73

purity

>95%

form

Lyophilized powder

Storage conditions

4°C for short term storage, -20°C for long term storage