FLAG peptide

Basic Description

​ ​Synthesis and Functionality​ ​:
FLAG Peptide is an artificially designed 8-amino acid short peptide (sequence: DYKDDDDK). As a highly specific epitope tag, it is widely used in affinity purification, localization and detection of recombinant proteins. Its core features include:
1. ​​High affinity​ ​：It can specifically bind to Anti-FLAG antibodies (such as M1/M2 monoclonal antibodies) and is suitable for Western Blot, immunoprecipitation (IP) and immunofluorescence (IF).
2. ​​Gentle elution​ ​：In the presence of calcium chelators (such as EDTA) or competitive FLAG peptides, mild elution conditions can be achieved to maintain the activity of the target protein.
3. ​Flexibility ​：Can be fused to the N-terminus, C-terminus or inside of the protein without affecting protein folding and function.

​ ​Application Areas​ ​:
• Recombinant protein purification (FLAG tag system)
• Protein interaction studies (Pull-down experiments)
• Cell localization and dynamic tracking (live cell imaging)

Complete sequence

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Simplify sequence

DYKDDDDK

Molecular formula

C₄₄H₆₂N₁₀O₁₅S

Molecular weight

1012.08

purity

>95%

form

Lyophilized powder

Storage conditions

Store in a dry place at -20℃