Pulmonary fibrosis is a chronic disease characterized by interstitial scarring and loss of function, which is common in idiopathic pulmonary fibrosis (IPF) and radiation/drug-induced lung injury. The following are the main experimental models and their construction methods, evaluation indicators and application scenarios.
Pulmonary fibrosis is a chronic disease characterized by interstitial scarring and loss of function, which is common in idiopathic pulmonary fibrosis (IPF) and radiation/drug-induced lung injury. The following are the main experimental models and their construction methods, evaluation indicators and application scenarios.
I. Model types and core methods
1. Chemical induction model (bleomycin-induced)
principle ： Bleomycin induces alveolar epithelial damage and activates fibroblast proliferation and collagen deposition by producing reactive oxygen species (ROS) and pro-inflammatory factors.
principle : Localized thoracic radiation (eg, single-fraction 10-20 Gy) causes DNA damage and chronic inflammation, simulating radiation-induced pulmonary fibrosis.
Applicable scenarios : To study the mechanism and intervention measures of pulmonary fibrosis after radiotherapy.
4. Cell transplantation model
Construction method :
Fibroblast adoptive transfer : In vitro activated fibroblasts (e.g. overexpressing α-SMA) were injected into mice via the tail vein.
Bone marrow derived cells (BMDC) : Transplantation of GFP-labeled BMDCs and tracking their differentiation in lung tissue (such as transformation into myofibroblasts).
Pain management: Administer ibuprofen or carprofen postoperatively.
End point criteria: euthanasia is required when body weight loss > 20% or respiratory distress occurs.
VI. Sample Data
Group
Ashcroft Rating
Hydroxyproline (μg/g)
Lung compliance (mL/cmH2O)
Control group
1.2 ± 0.3
12 ± 3
0.055 ± 0.005
Bleomycin model group
5.8 ± 1.1
42 ± 8
0.028 ± 0.003
Nintedanib treatment group
3.2 ± 0.7
25 ± 5
0.045 ± 0.004
Our company can provide customized synthesis of high-polypeptide, virtual screening of compounds, Animal disease model construction, Drug safety verification, Application technology support for gene editing animals, etc. For specific application consultation, please contact the sales department.
We look forward to your inquiries:
Sales Department 1: Manager Han Mobile phone: 18701856899
